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MBS1002 Biomedical Approaches

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Question:

What are next generations of Crispr/Cas9?

Author: Mirte Schattorie



Answer:

Base editors: derivatives of Crispr/Cas9 that do not make DSBs but can cause certain point mutations depending on the enzymes that are included in the base editor. enzymes can be cytidine deaminase (C>G) and adenine deaminase (A>T). prime editing: does not make DSBs, but can produce any precise edit or insertion by using reverse transcription. a peg RNA is used and it can be programmable to everything you want to do.


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