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MBS1002 Biomedical Approaches

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Question:

What are the different ways of physical transfection?

Author: Mirte Schattorie



Answer:

Creating holes in the cell membrane to allow for nucleic acid entry using: - electroporation: an electrical voltage is used to increase cell membrane permeability - sonoporation: microbubbles techniques - laser irritation-assisted transfection: a laser beam magnet-assisted transfection: a magnetic force is used to guide nucleic acids across the membrane gene microinjection: a needle is used to puncture the cell membrane and inject the nucleic acids into the nucleus.


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