B. Tube Method

1. Make a 3-5% RCS of both Patient (Px) and Donor (Do 2. Prepare 2 clean tubes and label with PX, DO respectively 3. Place 1 drop of anti-D typing sera on to each tube. 4. Add 1 drop of Patient RCS to tube PX and Donor RCS to tube DO. 5. Cover the tubes with Parafilm. 6. Mix the solution and spin for 15 seconds at 3,400 rpm. Gentle mixing is recommended. 7. Carefully dislodge the cell button by gently mixing it and examine for agglutination or hemolysis. 8. Grade the reaction and record the results.