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level: Microbiological Examination of Surfaces

Questions and Answers List

level questions: Microbiological Examination of Surfaces

QuestionAnswer
Swab method- used mostly on areas with uneven surfaces; cracks, corners, crevices, depressions, elbow joints - sterile cotton or calcium alginate swabs - flat area exposed by template is swabbed - inoculated swab placed in suitable diluent - vortexted to release microbes - SPC performed on inoculated diluent
Swab method: diluentsCotton swabs: 0.1% peptone, buffered peptone water, 0.85% saline Calcium alginate swabs soluble in: 1% sodium citrate, sodium glycerophosphate, sodium hexametaphosphate
Contact Plate Method (RODAC Method)- Replicate Organism Detection and Counting - used on smooth surfaces - best for cleaned & sanitized surfaces - highly contaminated surface --> overgrowth on agar plate - randomize selection of sampling sites - add neutralizers to agar to neutralize chemical sanitizer residues - plate with raised sterile agar medium - exposed agar pressed to flat surface - inoculated agar covered and incubated - bacterial colonies counted